| Titre : | Yeast diversity in honey bee hives and date fruits : Morphological characteristics and probiotic properties | | Type de document : | document multimédia | | Auteurs : | Fatima Zahra Senouci, Auteur ; Abderrahmane Houicher, Directeur de thèse ; Ahmed Ararem, Directeur de thèse | | Editeur : | Laghouat : Université Amar Telidji - Département d'agronomie | | Année de publication : | 2025 | | Importance : | 79 p. | | Accompagnement : | CD ROM | | Note générale : | Option :Agro alimentaire et controle de qualite | | Langues : | Anglais | | Mots-clés : | Yeast honey bee hive date fruit probiotic autoaggregation hydrophobicity gastrointestinal stress antimicrobial activity | | Résumé : | This scientific investigation aimed to isolate and partially identify yeasts from honey bee hives and date fruits, in order to uncover their probiotic potential for further applications. Morphological characterization based on selective isolation allowed identification of seven isolates belonging to four genera Hanseniaspora spp., Kluyveromyces spp., Pichia spp., and Saccharomyces spp. Physiological tests revealed that all isolates were capable to grow at 37 °C, tolerate acidic pH (2.5), and survive in bile salt concentration of 0.3%, indicating their potential to withstand gastrointestinal conditions. All strains presented a significant ability to autoaggregation (≈ 98%) and high hydrophobicity with the Pichia spp. isolate Da12 leading at 92%, promising intestinal adhesion potential. Moderate to high antimicrobial activity against Gram-positive and Gram-negative bacteria was observed in several isolates highlighting the isolate Hanseniaspora spp. Da18 with a strong inhibitory effect (19 mm). Importantly, safety evaluation confirmed the absence of hemolytic (γ-hemolysis) and gelatinase production in all isolates, while no production of proteolytic or lipolytic enzymes was detected. Our findings reveal the potential of these yeasts as candidates for probiotic applications in the food industry and/or as biocontrol agents in the agriculture sector. | | note de thèses : | Memoire de master des siences Agronomiques |
Yeast diversity in honey bee hives and date fruits : Morphological characteristics and probiotic properties [document multimédia] / Fatima Zahra Senouci, Auteur ; Abderrahmane Houicher, Directeur de thèse ; Ahmed Ararem, Directeur de thèse . - Laghouat : Université Amar Telidji - Département d'agronomie, 2025 . - 79 p. + CD ROM. Option :Agro alimentaire et controle de qualite Langues : Anglais | Mots-clés : | Yeast honey bee hive date fruit probiotic autoaggregation hydrophobicity gastrointestinal stress antimicrobial activity | | Résumé : | This scientific investigation aimed to isolate and partially identify yeasts from honey bee hives and date fruits, in order to uncover their probiotic potential for further applications. Morphological characterization based on selective isolation allowed identification of seven isolates belonging to four genera Hanseniaspora spp., Kluyveromyces spp., Pichia spp., and Saccharomyces spp. Physiological tests revealed that all isolates were capable to grow at 37 °C, tolerate acidic pH (2.5), and survive in bile salt concentration of 0.3%, indicating their potential to withstand gastrointestinal conditions. All strains presented a significant ability to autoaggregation (≈ 98%) and high hydrophobicity with the Pichia spp. isolate Da12 leading at 92%, promising intestinal adhesion potential. Moderate to high antimicrobial activity against Gram-positive and Gram-negative bacteria was observed in several isolates highlighting the isolate Hanseniaspora spp. Da18 with a strong inhibitory effect (19 mm). Importantly, safety evaluation confirmed the absence of hemolytic (γ-hemolysis) and gelatinase production in all isolates, while no production of proteolytic or lipolytic enzymes was detected. Our findings reveal the potential of these yeasts as candidates for probiotic applications in the food industry and/or as biocontrol agents in the agriculture sector. | | note de thèses : | Memoire de master des siences Agronomiques |
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Yeast diversity in honey bee hives and date fruits / Fatima Zahra Senouci
