Comparative analysis of the pathogenesis-related protein 10 gene family and their role during legume symbiosis / Toufik Yahia Takhi

Public ISBD Titre : Comparative analysis of the pathogenesis-related protein 10 gene family and their role during legume symbiosis Type de document : document multimédia Auteurs : Toufik Yahia Takhi, Auteur ; Fathi Berrabah, Directeur de thèse ; Véronique Gruber, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2024 Importance : 55 p. Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Microbiologie appliquée Langues : Anglais Mots-clés : PR10  Medicago  Nodule defense response  Nodule immunity  Symbiosis Résumé : Pathogenesis-related class 10 (PR10) proteins play various roles during plants’ growth and resistance to biotic and abiotic stress. However, little is known about their role in the context of legume-rhizobia symbiosis, particularly in nodules. This present study aims to bridge that gap by characterizing the various roles of PR10s in the nodules of Medicago truncatula (Medtr). Initially, phylogenetic analysis of PR10s revealed two distinct PR10 groups, PBI and PBII. The expression analysis performed on nodules of two Medtr mutants, symbiotic Cysteine-rich Kinase-Like (symCRK) and defective in nitrogen fixation 2 (dnf2), showed an upregulation of PR10s in symCRK and dnf2 mutant nodules compared to the WT, with PBI showing a higher expression than PBII. Collinearity analysis revealed three collinear PR10 gene pairs that all belong to PBI, suggesting that PR10s were possibly subjected to tandem gene duplication. The multiple sequence alignments of 500 nucleotides up-stream PR10 genes show a high conservation of 250 nucleotides in that site between PBI PR10 genes and a loss of this conservation in PBII PR10 genes. The conserved 250 up-stream nucleotides display a high density of putative binding sites for bZIP, NAC, and Myb transcription factors. Interestingly, molecular docking findings highlight that PBI PR10s bind phenolic compounds more efficiently than PBII. Moreover, multiple sequence alignments revealed that, unlike PBII PR10 proteins, PBI proteins have a high conservation of RNase motifs, which are important for the activation of programmed cell death during plant defenses. Finally, protein enrichment data revealed that PR10s can additionally accumulate in the different cellular compartments of nodules, including the cytoplasm, microsomes, and symbiosome. Altogether, our data show that PBI PR10s of M. truncatula are more conserved and highly functional during nodule defense responses compared to PBII PR10s. Thus, this study provides a valuable understanding of PR10s and their role during legume-rhizobia symbiosis. note de thèses : Mémoire de master en sciences biologiques Comparative analysis of the pathogenesis-related protein 10 gene family and their role during legume symbiosis [document multimédia] / Toufik Yahia Takhi, Auteur ; Fathi Berrabah, Directeur de thèse ; Véronique Gruber, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 55 p. + 1 disque optique numérique (CD-ROM). Option : Microbiologie appliquée Langues : Anglais Mots-clés : PR10  Medicago  Nodule defense response  Nodule immunity  Symbiosis Résumé : Pathogenesis-related class 10 (PR10) proteins play various roles during plants’ growth and resistance to biotic and abiotic stress. However, little is known about their role in the context of legume-rhizobia symbiosis, particularly in nodules. This present study aims to bridge that gap by characterizing the various roles of PR10s in the nodules of Medicago truncatula (Medtr). Initially, phylogenetic analysis of PR10s revealed two distinct PR10 groups, PBI and PBII. The expression analysis performed on nodules of two Medtr mutants, symbiotic Cysteine-rich Kinase-Like (symCRK) and defective in nitrogen fixation 2 (dnf2), showed an upregulation of PR10s in symCRK and dnf2 mutant nodules compared to the WT, with PBI showing a higher expression than PBII. Collinearity analysis revealed three collinear PR10 gene pairs that all belong to PBI, suggesting that PR10s were possibly subjected to tandem gene duplication. The multiple sequence alignments of 500 nucleotides up-stream PR10 genes show a high conservation of 250 nucleotides in that site between PBI PR10 genes and a loss of this conservation in PBII PR10 genes. The conserved 250 up-stream nucleotides display a high density of putative binding sites for bZIP, NAC, and Myb transcription factors. Interestingly, molecular docking findings highlight that PBI PR10s bind phenolic compounds more efficiently than PBII. Moreover, multiple sequence alignments revealed that, unlike PBII PR10 proteins, PBI proteins have a high conservation of RNase motifs, which are important for the activation of programmed cell death during plant defenses. Finally, protein enrichment data revealed that PR10s can additionally accumulate in the different cellular compartments of nodules, including the cytoplasm, microsomes, and symbiosome. Altogether, our data show that PBI PR10s of M. truncatula are more conserved and highly functional during nodule defense responses compared to PBII PR10s. Thus, this study provides a valuable understanding of PR10s and their role during legume-rhizobia symbiosis. note de thèses : Mémoire de master en sciences biologiques

Réservation

Réserver ce document

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MB 02-223	MB 02-223	CD	BIBLIOTHEQUE DE FACULTE DES SCIENCES	théses (sci)	Disponible

Identification and study of endophytic bacteria in the model plant Medicago truncatula / Fatima Zahra Djokhdem

Public ISBD Titre : Identification and study of endophytic bacteria in the model plant Medicago truncatula Type de document : document multimédia Auteurs : Fatima Zahra Djokhdem, Auteur ; Fathi Berrabah, Directeur de thèse ; Véronique Gruber, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2023 Importance : 50 p. Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Basic and applied microbiology (Microbiologie appliquée) Langues : Anglais Résumé : In this study, the effects of endophytes on the Medicago-Sinorhizobium symbiosis and the interaction between Medicago and endophyte bacteria were studied. For this, soil from organic farms or the desert was used to create a collection of endophytic bacterial strains. The capacity of 46 of them to infect Medicago R108 and A17 roots under axenic conditions was investigated. Five strains (M17, M18, M50, M67.2 and BT-37) among the forty validated bacteria were selected for molecular identification .We studied the potential effects of the PGPR of the strain studied, the analysis of the response of Medicago to salt stress in the presence or in the absence of endophytes was evaluated. The results indicate the presence of various effects of the tested strain on the protection of Medicago against abiotic stress. Interestingly, the less infectious strain may possess the highest protection against salt stress. the qPCR analysis of the PR10, PR5 and NDR1-Like genes after inoculation of the A17 ecotype by the endophytes showed Different levels of immune stimulation are observed depending on the strain used. Interestingly, the expressions of the PR10 genes are correlated with the level of tissue occupancy by endophytes .Finally, we have studied the impact of endophytes on the Medicago-Sinorhizobium symbiosis, a co-inoculation of Medicago with the strain studied and the rhizobium was carried out. Our preliminary data suggest that endophytes have a weak impact on the initiation of the symbiotic interaction. endophytes tested are able to infect nodules and reduce nitrogen-fixing capacity. Interestingly, Lysinibaccillus sp. These show the lowest root colonization ability, display the highest nodule infection rates. This observation underlies the difference between nodule and roots and could be explained by the difference in immunity and/or metabolic status. Finally we were able to demonstrate that infection rates do not seem to be correlated with nitrogen fixation. note de thèses : Mémoire de master en sciences biologiques Identification and study of endophytic bacteria in the model plant Medicago truncatula [document multimédia] / Fatima Zahra Djokhdem, Auteur ; Fathi Berrabah, Directeur de thèse ; Véronique Gruber, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2023 . - 50 p. + 1 disque optique numérique (CD-ROM). Option : Basic and applied microbiology (Microbiologie appliquée) Langues : Anglais Résumé : In this study, the effects of endophytes on the Medicago-Sinorhizobium symbiosis and the interaction between Medicago and endophyte bacteria were studied. For this, soil from organic farms or the desert was used to create a collection of endophytic bacterial strains. The capacity of 46 of them to infect Medicago R108 and A17 roots under axenic conditions was investigated. Five strains (M17, M18, M50, M67.2 and BT-37) among the forty validated bacteria were selected for molecular identification .We studied the potential effects of the PGPR of the strain studied, the analysis of the response of Medicago to salt stress in the presence or in the absence of endophytes was evaluated. The results indicate the presence of various effects of the tested strain on the protection of Medicago against abiotic stress. Interestingly, the less infectious strain may possess the highest protection against salt stress. the qPCR analysis of the PR10, PR5 and NDR1-Like genes after inoculation of the A17 ecotype by the endophytes showed Different levels of immune stimulation are observed depending on the strain used. Interestingly, the expressions of the PR10 genes are correlated with the level of tissue occupancy by endophytes .Finally, we have studied the impact of endophytes on the Medicago-Sinorhizobium symbiosis, a co-inoculation of Medicago with the strain studied and the rhizobium was carried out. Our preliminary data suggest that endophytes have a weak impact on the initiation of the symbiotic interaction. endophytes tested are able to infect nodules and reduce nitrogen-fixing capacity. Interestingly, Lysinibaccillus sp. These show the lowest root colonization ability, display the highest nodule infection rates. This observation underlies the difference between nodule and roots and could be explained by the difference in immunity and/or metabolic status. Finally we were able to demonstrate that infection rates do not seem to be correlated with nitrogen fixation. note de thèses : Mémoire de master en sciences biologiques

Réservation

Réserver ce document

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MB 02-202	MB 02-202	CD	BIBLIOTHEQUE DE FACULTE DES SCIENCES	théses (sci)	Disponible

Protection of Alfalfa from salt stress using Endophytic bacteria / Rania Berkai

Public ISBD Titre : Protection of Alfalfa from salt stress using Endophytic bacteria Type de document : document multimédia Auteurs : Rania Berkai, Auteur ; Fathi Berrabah, Directeur de thèse ; Asma Rezzoug, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2024 Importance : 54 p. Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Microbiologie appliquée Langues : Anglais Mots-clés : Plant microbiome  Salt stress  Endophytic bacteria  PGPR  Genes Résumé : Endophytes are microorganisms mostly bacteria present asymptomatically in plants and can exercise several beneficial activities for their hosts. There are many reports currently that microorganisms isolated from the extreme environments have great biotechnological applications in medicine, agriculture, and industry. Alfalfa is a fodder legume of great S116) were studied for their ability to reduce Medicago sativa salt stress. Results show different levels in responses of M. sativa alfalfa. Genomic analyses were performed using selectedeconomic importance, but its productivity is often affected by salt stress in arid and semi-arid regions. Endophytic bacteria, which colonize the internal tissues of plants in a symbiotic manner, represent a promising solution for improving the salt tolerance of alfalfa. To improve alfalfa salt stress resistance, four endophytic bacteria (M17, M50, S113 and strains M17 that show a good protection from salt stress. M17 is identified as Pseudomonas aeruginosastrain, Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): two strains associated with plants and two non-associated with plant, one phytopathogens and Human pathogen). The results shows 521 common genes and synteny analyses revelas a high conservation between the bacteria associated with plants. Pseudomonas which indicate a mechanism to protect plants from salt and other abiotic and biotic stresses. note de thèses : Mémoire de master en sciences biologiques Protection of Alfalfa from salt stress using Endophytic bacteria [document multimédia] / Rania Berkai, Auteur ; Fathi Berrabah, Directeur de thèse ; Asma Rezzoug, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 54 p. + 1 disque optique numérique (CD-ROM). Option : Microbiologie appliquée Langues : Anglais Mots-clés : Plant microbiome  Salt stress  Endophytic bacteria  PGPR  Genes Résumé : Endophytes are microorganisms mostly bacteria present asymptomatically in plants and can exercise several beneficial activities for their hosts. There are many reports currently that microorganisms isolated from the extreme environments have great biotechnological applications in medicine, agriculture, and industry. Alfalfa is a fodder legume of great S116) were studied for their ability to reduce Medicago sativa salt stress. Results show different levels in responses of M. sativa alfalfa. Genomic analyses were performed using selectedeconomic importance, but its productivity is often affected by salt stress in arid and semi-arid regions. Endophytic bacteria, which colonize the internal tissues of plants in a symbiotic manner, represent a promising solution for improving the salt tolerance of alfalfa. To improve alfalfa salt stress resistance, four endophytic bacteria (M17, M50, S113 and strains M17 that show a good protection from salt stress. M17 is identified as Pseudomonas aeruginosastrain, Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): two strains associated with plants and two non-associated with plant, one phytopathogens and Human pathogen). The results shows 521 common genes and synteny analyses revelas a high conservation between the bacteria associated with plants. Pseudomonas which indicate a mechanism to protect plants from salt and other abiotic and biotic stresses. note de thèses : Mémoire de master en sciences biologiques

Réservation

Réserver ce document

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MB 02-216	MB 02-216	CD	BIBLIOTHEQUE DE FACULTE DES SCIENCES	théses (sci)	Disponible

Study of Arabidopsis thaliana response to salt stress and endophytic bacteria / Hanane Benferhat

Public ISBD Titre : Study of Arabidopsis thaliana response to salt stress and endophytic bacteria Type de document : document multimédia Auteurs : Hanane Benferhat, Auteur ; Fathi Berrabah, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2024 Importance : 73 p. Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Microbiologie appliquée Langues : Anglais Mots-clés : A. thaliana  M. sativa  salt stress  endophytic bacteria  PGPR  genes Résumé : Plants can shape an amazing area around their roots, through releasing a plethora of chemical molecules (root exudates) such as sugar, amino acid and secondary metabolites, that are representing a high part of their photosynthesis process. These molecules select mostly beneficial microbes to interact in positive manner leading to making up the second genome of plant a unique entity called holobiont. Even that plants are making healthy relationships, it does not mean that are safe. Soil salinization is one of the biggest challenging facing the development of crops that are considered as glycophytic. Endophytic bacteria provide an eco-friendly and cost-effective natural solution to alleviate salt stress. Five bacterial strains are used to perform this study about Arabidopsis thaliana response in salt stress and endophytic bacteria. The seeds of Arabidopsis are sterilized in SDS+70% ethanol, then inoculated with bacterial suspension. After germination, seedlings are incubated in the growth chamber for 10 days. Medicago alfalfa seedlings are used to confirm the efficacy of endophytes. Results show different levels in responses between both inoculated Arabidopsis and Alfalfa. Genomic analyses were performed using selected strains M17 and M67.2 that show a significant protection from salt stress. M17 is identified as Pseudomonas aeruginosa strain where M67.2 is Bacillus amyloliquefaciens. Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): three strains associated with plants, phytopathogen and Human pathogen) shows 1427 common genes where four strains are used for M67.2 which are three strains associated with plants and one non associated with plant. Syntenic analyses were performed between these strains using the largest genome (Pseudomonas fluorescens F113) as a reference and the closest strain (Bacillus velezensis FZB42) for M67.2. The results indicate a high conservation between the bacteria associated with plants in both strains. Genes identification is used to understand the complex network associated with plants. It shows common genes between the two genera. Protein-protein interaction is generated by STRING database for seven genes potentially involved in the protection mechanism; (cat, opuAB, betB, bcp, katA, tpx, gbsB). A complex interplay of a multitude of genes mediates salt resistance. note de thèses : Mémoire de master en sciences biologiques Study of Arabidopsis thaliana response to salt stress and endophytic bacteria [document multimédia] / Hanane Benferhat, Auteur ; Fathi Berrabah, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 73 p. + 1 disque optique numérique (CD-ROM). Option : Microbiologie appliquée Langues : Anglais Mots-clés : A. thaliana  M. sativa  salt stress  endophytic bacteria  PGPR  genes Résumé : Plants can shape an amazing area around their roots, through releasing a plethora of chemical molecules (root exudates) such as sugar, amino acid and secondary metabolites, that are representing a high part of their photosynthesis process. These molecules select mostly beneficial microbes to interact in positive manner leading to making up the second genome of plant a unique entity called holobiont. Even that plants are making healthy relationships, it does not mean that are safe. Soil salinization is one of the biggest challenging facing the development of crops that are considered as glycophytic. Endophytic bacteria provide an eco-friendly and cost-effective natural solution to alleviate salt stress. Five bacterial strains are used to perform this study about Arabidopsis thaliana response in salt stress and endophytic bacteria. The seeds of Arabidopsis are sterilized in SDS+70% ethanol, then inoculated with bacterial suspension. After germination, seedlings are incubated in the growth chamber for 10 days. Medicago alfalfa seedlings are used to confirm the efficacy of endophytes. Results show different levels in responses between both inoculated Arabidopsis and Alfalfa. Genomic analyses were performed using selected strains M17 and M67.2 that show a significant protection from salt stress. M17 is identified as Pseudomonas aeruginosa strain where M67.2 is Bacillus amyloliquefaciens. Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): three strains associated with plants, phytopathogen and Human pathogen) shows 1427 common genes where four strains are used for M67.2 which are three strains associated with plants and one non associated with plant. Syntenic analyses were performed between these strains using the largest genome (Pseudomonas fluorescens F113) as a reference and the closest strain (Bacillus velezensis FZB42) for M67.2. The results indicate a high conservation between the bacteria associated with plants in both strains. Genes identification is used to understand the complex network associated with plants. It shows common genes between the two genera. Protein-protein interaction is generated by STRING database for seven genes potentially involved in the protection mechanism; (cat, opuAB, betB, bcp, katA, tpx, gbsB). A complex interplay of a multitude of genes mediates salt resistance. note de thèses : Mémoire de master en sciences biologiques

Réservation

Réserver ce document

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MB 02-214	MB 02-214	CD	BIBLIOTHEQUE DE FACULTE DES SCIENCES	théses (sci)	Disponible

Study of Arabidopsis thaliana salt stress protection by beneficial bacteria / Nour Elhorra Bentria

Public ISBD Titre : Study of Arabidopsis thaliana salt stress protection by beneficial bacteria Type de document : document multimédia Auteurs : Nour Elhorra Bentria, Auteur ; Fathi Berrabah, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2024 Importance : 32 p. Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Microbiologie appliquée Langues : Anglais Mots-clés : A. thaliana  SA  salt stress  endophytic bacteria  npr1  NohG Résumé : Salinity is one of the world's major issues affecting soil quality and agricultural productivity. Some plant growth-promoting bacteria that can thrive in regions with high salt concentrations (NaCl) biotic and abiotic stress have the ability to promote plant growth in salty environments. Arabidopsis thaliana is a model plants giving a precious help for understanding the plant sensibility and resistance to the salt stress. In this study we harvest A. thaliana sensibility to salt stress, we assess the role of the defense hormone salicylic acid (SA) in this response by the study of plant overexpression SA-degrading enzyme (NohG) or impaired in SA signaling (nrp1). Our results indicate that the NaCL at 100mM is sufficient to inhibit the growth of A. thaliana cultivated in vitro. Interestingly, 35S::NohG shows increase of the plant growth in absence of salt stress and increase of sensitivity to the NaCl. By contrast the mutant npr1 show similar behavior with the WT in absence or presence of NaCl. Four Medicago truncatula bacterial endophytes (M17, M50, S113 and S116) were tested for their ability to reduce the salt stress on A. thaliana. The strain S116 increase the resistance and the growth of A. thaliana in response to salt stress, wherase the other strain shows reduction of the impact of the NaCl on the plant growth. Inoculation of 35S::NohG and npr-1 with the studied strain reveals that the resistance mediated by the bacteria required correct SA synthesis and signaling. Finally comparative genomic analysis reveals the presence of shikimate kinase and salicylate esterase in M50 closest strain suggesting a potential manipulation of the SA pathway for the increase of A. thaliana resistance to the salt stress. note de thèses : Mémoire de master en sciences biologiques Study of Arabidopsis thaliana salt stress protection by beneficial bacteria [document multimédia] / Nour Elhorra Bentria, Auteur ; Fathi Berrabah, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 32 p. + 1 disque optique numérique (CD-ROM). Option : Microbiologie appliquée Langues : Anglais Mots-clés : A. thaliana  SA  salt stress  endophytic bacteria  npr1  NohG Résumé : Salinity is one of the world's major issues affecting soil quality and agricultural productivity. Some plant growth-promoting bacteria that can thrive in regions with high salt concentrations (NaCl) biotic and abiotic stress have the ability to promote plant growth in salty environments. Arabidopsis thaliana is a model plants giving a precious help for understanding the plant sensibility and resistance to the salt stress. In this study we harvest A. thaliana sensibility to salt stress, we assess the role of the defense hormone salicylic acid (SA) in this response by the study of plant overexpression SA-degrading enzyme (NohG) or impaired in SA signaling (nrp1). Our results indicate that the NaCL at 100mM is sufficient to inhibit the growth of A. thaliana cultivated in vitro. Interestingly, 35S::NohG shows increase of the plant growth in absence of salt stress and increase of sensitivity to the NaCl. By contrast the mutant npr1 show similar behavior with the WT in absence or presence of NaCl. Four Medicago truncatula bacterial endophytes (M17, M50, S113 and S116) were tested for their ability to reduce the salt stress on A. thaliana. The strain S116 increase the resistance and the growth of A. thaliana in response to salt stress, wherase the other strain shows reduction of the impact of the NaCl on the plant growth. Inoculation of 35S::NohG and npr-1 with the studied strain reveals that the resistance mediated by the bacteria required correct SA synthesis and signaling. Finally comparative genomic analysis reveals the presence of shikimate kinase and salicylate esterase in M50 closest strain suggesting a potential manipulation of the SA pathway for the increase of A. thaliana resistance to the salt stress. note de thèses : Mémoire de master en sciences biologiques

Réservation

Réserver ce document

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MB 02-215	MB 02-215	CD	BIBLIOTHEQUE DE FACULTE DES SCIENCES	théses (sci)	Disponible

Understanding phenols function during tripartite interaction between legume- rhizobia and endophytic bacteria / Maroua Makhloufi

Permalink

---

page 1/1