Titre :	Techniques for the isolation and identification of haloterant Penicillium species fdrom saline terrestrial environments
Type de document :	texte manuscrit
Auteurs :	Souhila Rouane, Auteur ; Zahra Zaabane, Auteur ; Djalila Takhi, Directeur de thèse
Editeur :	Laghouat : Université Amar Telidji - Département de biologie
Année de publication :	2020
Importance :	38 p.
Format :	30 cm.
Accompagnement :	1 disque optique numérique (CD-ROM)
Note générale :	Option : Applied microbiology (Microbiologie appliquée)
Langues :	Anglais
Mots-clés :	Penicillium  Haloterance  Saline soil  Fungal diversity  Halotérance  Milieux terrestres salins  Diversité fongique
Résumé :	Penicillium is a well-characterized fungus found in a diverse range of habitats especially in soil. This genus is one of the dominant mycobiota in hypersaline terrestrial environment. Traditionally, morphological identification methods were used to identify fungi such as Penicillium. However, with the emergence of more accurate and rapid molecular identification tools, scientists embraced the polyphasic approach to address fungal diversity study challenges. The aim of our study is presenting a summary of isolation and identification techniques of halotolerant species of Penicillium as well as a preview on their diversity in some saline terrestrial habitats. From all the studies analyzed, 67 isolates were distributed on 47 different species that have been isolated from 11 different regions of saline soils, in which these regions have different physicochemical properties. The distribution of different Penicillium species varies from a region to another. The species variability is likely due to the variation in physicochemical characteristics of each studied habitat, such as pH, salinity, temperature… etc. The isolated Penicillium species exhibited different tolerance levels to these parameters. Most of the isolated species were alkalotolerant (67.19%), whereas some were acidotolerant (32.81%). All of the isolates were halotolerant tolerating up to 20% NaCl, and a few could tolerate up to 22% of NaCl on solid medium. However, these isolates were unable to grow in salinity beyond 15% NaCl in liquid cultures. In all of the cited studies, Penicillium was one of the most dominant genera, with P. chrysogenum as the most abundant specie (7.46%). Four studies about non-saline soils were used as an element of comparison. These soils shared 22 similar species (46.81%) with those found in the explored saline soils. Penicillium species can enhance sporulation and produce various secondary metabolites to adapt to extreme environmental conditions. Furthermore, the diversity of micromycetes community in hypersaline habitats is more dependent on the physicochemical parameters of habitat than on the geographical location of their habitat. The species inhabiting extreme environments can be used as unique sources of enzymes or secondary metabolites, tolerant to extreme conditions, with a biotechnological utility.
note de thèses :	Mémoire de master en sciences biologiques

Techniques for the isolation and identification of haloterant Penicillium species fdrom saline terrestrial environments [texte manuscrit] / Souhila Rouane, Auteur ; Zahra Zaabane, Auteur ; Djalila Takhi, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2020 . - 38 p. ; 30 cm. + 1 disque optique numérique (CD-ROM).
Option : Applied microbiology (Microbiologie appliquée)
Langues : Anglais

Mots-clés :	Penicillium  Haloterance  Saline soil  Fungal diversity  Halotérance  Milieux terrestres salins  Diversité fongique
Résumé :	Penicillium is a well-characterized fungus found in a diverse range of habitats especially in soil. This genus is one of the dominant mycobiota in hypersaline terrestrial environment. Traditionally, morphological identification methods were used to identify fungi such as Penicillium. However, with the emergence of more accurate and rapid molecular identification tools, scientists embraced the polyphasic approach to address fungal diversity study challenges. The aim of our study is presenting a summary of isolation and identification techniques of halotolerant species of Penicillium as well as a preview on their diversity in some saline terrestrial habitats. From all the studies analyzed, 67 isolates were distributed on 47 different species that have been isolated from 11 different regions of saline soils, in which these regions have different physicochemical properties. The distribution of different Penicillium species varies from a region to another. The species variability is likely due to the variation in physicochemical characteristics of each studied habitat, such as pH, salinity, temperature… etc. The isolated Penicillium species exhibited different tolerance levels to these parameters. Most of the isolated species were alkalotolerant (67.19%), whereas some were acidotolerant (32.81%). All of the isolates were halotolerant tolerating up to 20% NaCl, and a few could tolerate up to 22% of NaCl on solid medium. However, these isolates were unable to grow in salinity beyond 15% NaCl in liquid cultures. In all of the cited studies, Penicillium was one of the most dominant genera, with P. chrysogenum as the most abundant specie (7.46%). Four studies about non-saline soils were used as an element of comparison. These soils shared 22 similar species (46.81%) with those found in the explored saline soils. Penicillium species can enhance sporulation and produce various secondary metabolites to adapt to extreme environmental conditions. Furthermore, the diversity of micromycetes community in hypersaline habitats is more dependent on the physicochemical parameters of habitat than on the geographical location of their habitat. The species inhabiting extreme environments can be used as unique sources of enzymes or secondary metabolites, tolerant to extreme conditions, with a biotechnological utility.
note de thèses :	Mémoire de master en sciences biologiques