| Titre : | Antimicrobial activity of natural extracts from spontaneous plants in comparison with some synthetic compounds | | Type de document : | document multimédia | | Auteurs : | Siham Nahoui, Auteur ; Houria Saci, Auteur ; Hadjer Boussoussa, Directeur de thèse ; Ihcen Khacheba, Directeur de thèse | | Editeur : | Laghouat : Université Amar Telidji - Département de biologie | | Année de publication : | 2024 | | Importance : | 82 p. | | Accompagnement : | 1 disque optique numérique (CD-ROM) | | Note générale : | Option : Biochimie appliquée | | Langues : | Anglais | | Mots-clés : | antimicrobial activity natural extracts essential oils Inhibition diameter MIC MBC | | Résumé : | This master thesis aimed to evaluate and compare the antimicrobial activity of some synthetic compounds with natural methanolic and essential oils extracts of cloves, cinnamon and lavender. The adopted methodology combines laboratory tests against different pathogenic bacterial strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans).
The extracts were tested using disk diffusion and microdilution methods, and the results appeared as follows:
As for the essential oils, the disc diffusion results showed different diameters of inhibition ranging between 30mm and 20mm for the essential oil of cloves and between 45mm and 28mm for the essential oil of cinnamon with the various strains used. We recorded the highest diameters of inhibition equal to 50mm for the pure essential oil of cloves with Candida albicans ,and the pure essential oil for cinnamon with Escherichia coli. As for the antibiotic gentamicin, its inhibition diameter is equal to 20 mm with the various strains used. As for the natural and synthetic extracts, the results of microdilution showed that the MIC of the cinnamon extract diluted 1/2 is equal to 0.475 mg/ml with Escherichia coli, and the MIC of the pure lavender extract is equal to 1.49 mg/ml with Candida albicans. While the MIC of the synthetic extract Ascorbic acid is equal to 1.59mg/ml with Staphylococcus aureus. From this, we conclude that natural extracts of cinnamon, lavender, and volatile oils of cloves and cinnamon have significant antimicrobial activity, even greater than synthetic extracts and the antibiotic gentamicin.
In conclusion, this study highlights the potential of natural extracts and essential oils as promising alternatives to synthetic antimicrobial agents. | | note de thèses : | Mémoire de master en sciences biologiques |
Antimicrobial activity of natural extracts from spontaneous plants in comparison with some synthetic compounds [document multimédia] / Siham Nahoui, Auteur ; Houria Saci, Auteur ; Hadjer Boussoussa, Directeur de thèse ; Ihcen Khacheba, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 82 p. + 1 disque optique numérique (CD-ROM). Option : Biochimie appliquée Langues : Anglais | Mots-clés : | antimicrobial activity natural extracts essential oils Inhibition diameter MIC MBC | | Résumé : | This master thesis aimed to evaluate and compare the antimicrobial activity of some synthetic compounds with natural methanolic and essential oils extracts of cloves, cinnamon and lavender. The adopted methodology combines laboratory tests against different pathogenic bacterial strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans).
The extracts were tested using disk diffusion and microdilution methods, and the results appeared as follows:
As for the essential oils, the disc diffusion results showed different diameters of inhibition ranging between 30mm and 20mm for the essential oil of cloves and between 45mm and 28mm for the essential oil of cinnamon with the various strains used. We recorded the highest diameters of inhibition equal to 50mm for the pure essential oil of cloves with Candida albicans ,and the pure essential oil for cinnamon with Escherichia coli. As for the antibiotic gentamicin, its inhibition diameter is equal to 20 mm with the various strains used. As for the natural and synthetic extracts, the results of microdilution showed that the MIC of the cinnamon extract diluted 1/2 is equal to 0.475 mg/ml with Escherichia coli, and the MIC of the pure lavender extract is equal to 1.49 mg/ml with Candida albicans. While the MIC of the synthetic extract Ascorbic acid is equal to 1.59mg/ml with Staphylococcus aureus. From this, we conclude that natural extracts of cinnamon, lavender, and volatile oils of cloves and cinnamon have significant antimicrobial activity, even greater than synthetic extracts and the antibiotic gentamicin.
In conclusion, this study highlights the potential of natural extracts and essential oils as promising alternatives to synthetic antimicrobial agents. | | note de thèses : | Mémoire de master en sciences biologiques |
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