| Titre : | Study of Arabidopsis thaliana response to salt stress and endophytic bacteria | | Type de document : | document multimédia | | Auteurs : | Hanane Benferhat, Auteur ; Fathi Berrabah, Directeur de thèse | | Editeur : | Laghouat : Université Amar Telidji - Département de biologie | | Année de publication : | 2024 | | Importance : | 73 p. | | Accompagnement : | 1 disque optique numérique (CD-ROM) | | Note générale : | Option : Microbiologie appliquée | | Langues : | Anglais | | Mots-clés : | A. thaliana M. sativa salt stress endophytic bacteria PGPR genes | | Résumé : | Plants can shape an amazing area around their roots, through releasing a plethora of chemical molecules (root exudates) such as sugar, amino acid and secondary metabolites, that are representing a high part of their photosynthesis process. These molecules select mostly beneficial microbes to interact in positive manner leading to making up the second genome of plant a unique entity called holobiont. Even that plants are making healthy relationships, it does not mean that are safe. Soil salinization is one of the biggest challenging facing the development of crops that are considered as glycophytic. Endophytic bacteria provide an eco-friendly and cost-effective natural solution to alleviate salt stress. Five bacterial strains are used to perform this study about Arabidopsis thaliana response in salt stress and endophytic bacteria. The seeds of Arabidopsis are sterilized in SDS+70% ethanol, then inoculated with bacterial suspension. After germination, seedlings are incubated in the growth chamber for 10 days. Medicago alfalfa seedlings are used to confirm the efficacy of endophytes. Results show different levels in responses between both inoculated Arabidopsis and Alfalfa. Genomic analyses were performed using selected strains M17 and M67.2 that show a significant protection from salt stress. M17 is identified as Pseudomonas aeruginosa strain where M67.2 is Bacillus amyloliquefaciens. Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): three strains associated with plants, phytopathogen and Human pathogen) shows 1427 common genes where four strains are used for M67.2 which are three strains associated with plants and one non associated with plant. Syntenic analyses were performed between these strains using the largest genome (Pseudomonas fluorescens F113) as a reference and the closest strain (Bacillus velezensis FZB42) for M67.2. The results indicate a high conservation between the bacteria associated with plants in both strains. Genes identification is used to understand the complex network associated with plants. It shows common genes between the two genera. Protein-protein interaction is generated by STRING database for seven genes potentially involved in the protection mechanism; (cat, opuAB, betB, bcp, katA, tpx, gbsB). A complex interplay of a multitude of genes mediates salt resistance. | | note de thèses : | Mémoire de master en sciences biologiques |
Study of Arabidopsis thaliana response to salt stress and endophytic bacteria [document multimédia] / Hanane Benferhat, Auteur ; Fathi Berrabah, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 73 p. + 1 disque optique numérique (CD-ROM). Option : Microbiologie appliquée Langues : Anglais | Mots-clés : | A. thaliana M. sativa salt stress endophytic bacteria PGPR genes | | Résumé : | Plants can shape an amazing area around their roots, through releasing a plethora of chemical molecules (root exudates) such as sugar, amino acid and secondary metabolites, that are representing a high part of their photosynthesis process. These molecules select mostly beneficial microbes to interact in positive manner leading to making up the second genome of plant a unique entity called holobiont. Even that plants are making healthy relationships, it does not mean that are safe. Soil salinization is one of the biggest challenging facing the development of crops that are considered as glycophytic. Endophytic bacteria provide an eco-friendly and cost-effective natural solution to alleviate salt stress. Five bacterial strains are used to perform this study about Arabidopsis thaliana response in salt stress and endophytic bacteria. The seeds of Arabidopsis are sterilized in SDS+70% ethanol, then inoculated with bacterial suspension. After germination, seedlings are incubated in the growth chamber for 10 days. Medicago alfalfa seedlings are used to confirm the efficacy of endophytes. Results show different levels in responses between both inoculated Arabidopsis and Alfalfa. Genomic analyses were performed using selected strains M17 and M67.2 that show a significant protection from salt stress. M17 is identified as Pseudomonas aeruginosa strain where M67.2 is Bacillus amyloliquefaciens. Comparative genomic for both strains is illustrated. For Pseudomonas strains (M17): three strains associated with plants, phytopathogen and Human pathogen) shows 1427 common genes where four strains are used for M67.2 which are three strains associated with plants and one non associated with plant. Syntenic analyses were performed between these strains using the largest genome (Pseudomonas fluorescens F113) as a reference and the closest strain (Bacillus velezensis FZB42) for M67.2. The results indicate a high conservation between the bacteria associated with plants in both strains. Genes identification is used to understand the complex network associated with plants. It shows common genes between the two genera. Protein-protein interaction is generated by STRING database for seven genes potentially involved in the protection mechanism; (cat, opuAB, betB, bcp, katA, tpx, gbsB). A complex interplay of a multitude of genes mediates salt resistance. | | note de thèses : | Mémoire de master en sciences biologiques |
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