| Titre : | Chemical composition of Deverra scoparia Coss. & Durieu essential oils and their phenolic extracts antioxidant activity | | Type de document : | texte manuscrit | | Auteurs : | Adel Ben Arfa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Yousfi, Directeur de thèse | | Editeur : | Laghouat : Université Amar Telidji - Département des sciences de la matière | | Année de publication : | 2021 | | Importance : | 136 p | | Format : | 27 cm | | Accompagnement : | 1 disque optique numérique (CD-ROM) | | Note générale : | Option : Applied organic chemistry | | Langues : | Anglais | | Catégories : | THESES :06 chimie
| | Mots-clés : | Deverra scoparia Hydro-distillation Essential oils Phenolic extracts Ultrasound-assisted extraction Response surface methodology Antioxidant activity | | Résumé : | The widespread use of Deverra scoparia plant (Apiaceous family) in Algerian folk medicine as a remedy, can be relatively attributed to the content of its secondary metabolites. The current study aimed to provide a scientific basis for the potential uses (applications) of this plant extracts in foods (natural antioxidants) and in pharmaceutics industries. This investigation was applied using a large number of randomly collected samples (large scale statistical analysis study) from different regions and locations spreading from semiarid to Saharan territories in Algeria, during two consecutive years (2016 and 2017). Essential oils (EOs) were obtained by hydro-distillation of different parts (flowers and stems) of D. scoparia. EO components were identified using GC and GC/MS. Results showed a considerable variation in the essential oil yields for both studied parts. For the stems part, the EO yield was moderate and varied from 0.48% to 1.68% (vol/wt), while for flowers part it was considerably important and varied in the range of 1.0–4.87% (vol/wt). Seven major compounds identified for the flowers part were varying from very low to very high percentages: α-pinene (2.49–42.32%), sabinene + β-pinene (1.11–30.31%), α-phellandrene (0.99–20.63%), p-cymene (0.0–44.22%), limonene (0.0–42.99%), myristicin (0.03–58.19%) and dill apiole (0.4–25.52%). Similarly, for the stems part, larges variations of both major and minor components were also registered: α-pinene (17.97–53.25%), sabinene + β-pinene (3.44–24.99%), α-phellandrene (0.75–10.32%), limonene (1.35–59.68%), terpinen-4-ol (0.29–15.85%) and spathulenol (0.46–11.29%). Both phenylpropanoids and phthalides classes of bioactive components in leaves part were exhibiting drastic variations: (0.77–59.46%) and (0.04–28.55%), respectively. More specifically, the following bioactive components reached a very high level for this plant species: myristicin “up to 58.19%”, dill apiole “up to 25.52%” and (Z)-ligustilide “up to 27.5%”. Agglomerative Hierarchical Cluster analysis was conducted, showing the existence of two main groups. Obtained data for volatile components in each plant part gave an insight of the existence of clear and large biodiversity of the chemical profiles of the studied plant parts. The second part of this work was devoted to the phenolic extracts of the flowers part using methanolic solvent. First step, an ultrasound-assisted extraction (UAE) using response surface methodology (RSM) was conducted to optimize the three factors, namely the extraction period, temperature, and sample-to-methanol ratio, on five responses, which were: extraction yield (Y%), Total phenolic content (TPC), Flavonoids content (FC) and two antioxidant activities tests DPPH and FRAP. The optimum conditions were: 50°C for 55 min using sample-to-methanol ratio of 1:70 g/mL (wt/vol). Experimental values were in agreement with the predicted ones, indicating the capability of the model and succeed of RSM in optimizing the extraction conditions. In the second step, the different phenolic classes of the methanolic extracts (TPC, FC and CTC) were quantified at the optimized determined conditions using spectroscopic methods. Subsequently, these extracts were also investigated for their in vitro antioxidant activities using an extended number of assays, namely: DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests. Correlations between these phenolic extracts classes and antioxidant activities were also investigated using Pearson method at level of significance of 0.05.
The quantification of the phenolic extracts results, revealed considerable amounts and variability of TPC (804-544 mg GAE/100 g dm, FC (187-410 mg QE/100 g dm) and CTC (111-394 mg CE/100 g dm). For the antioxidant activities, the most active extracts were corresponding to the following EC50 values of 0.057, 0.005, 0.021, 0.053 and 0.078 (g/L), for the DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests, respectively. Finally, the correlations results showed good correlation between TPC and FC (R2 = 72.49%). Moreover, high correlations were mostly observed between CUPRAC and DPPH, FRAP tests. Finally, this study provided certainly some additional and valuable data about the phytochemical and the chemosystematic characteristics of this plant, which should be useful for the subsequent studies on the pharmacological promising effects of its extracts. | | note de thèses : | Thèse de doctorat en chimie |
Chemical composition of Deverra scoparia Coss. & Durieu essential oils and their phenolic extracts antioxidant activity [texte manuscrit] / Adel Ben Arfa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Yousfi, Directeur de thèse . - Laghouat : Université Amar Telidji - Département des sciences de la matière, 2021 . - 136 p ; 27 cm + 1 disque optique numérique (CD-ROM). Option : Applied organic chemistry Langues : Anglais | Catégories : | THESES :06 chimie
| | Mots-clés : | Deverra scoparia Hydro-distillation Essential oils Phenolic extracts Ultrasound-assisted extraction Response surface methodology Antioxidant activity | | Résumé : | The widespread use of Deverra scoparia plant (Apiaceous family) in Algerian folk medicine as a remedy, can be relatively attributed to the content of its secondary metabolites. The current study aimed to provide a scientific basis for the potential uses (applications) of this plant extracts in foods (natural antioxidants) and in pharmaceutics industries. This investigation was applied using a large number of randomly collected samples (large scale statistical analysis study) from different regions and locations spreading from semiarid to Saharan territories in Algeria, during two consecutive years (2016 and 2017). Essential oils (EOs) were obtained by hydro-distillation of different parts (flowers and stems) of D. scoparia. EO components were identified using GC and GC/MS. Results showed a considerable variation in the essential oil yields for both studied parts. For the stems part, the EO yield was moderate and varied from 0.48% to 1.68% (vol/wt), while for flowers part it was considerably important and varied in the range of 1.0–4.87% (vol/wt). Seven major compounds identified for the flowers part were varying from very low to very high percentages: α-pinene (2.49–42.32%), sabinene + β-pinene (1.11–30.31%), α-phellandrene (0.99–20.63%), p-cymene (0.0–44.22%), limonene (0.0–42.99%), myristicin (0.03–58.19%) and dill apiole (0.4–25.52%). Similarly, for the stems part, larges variations of both major and minor components were also registered: α-pinene (17.97–53.25%), sabinene + β-pinene (3.44–24.99%), α-phellandrene (0.75–10.32%), limonene (1.35–59.68%), terpinen-4-ol (0.29–15.85%) and spathulenol (0.46–11.29%). Both phenylpropanoids and phthalides classes of bioactive components in leaves part were exhibiting drastic variations: (0.77–59.46%) and (0.04–28.55%), respectively. More specifically, the following bioactive components reached a very high level for this plant species: myristicin “up to 58.19%”, dill apiole “up to 25.52%” and (Z)-ligustilide “up to 27.5%”. Agglomerative Hierarchical Cluster analysis was conducted, showing the existence of two main groups. Obtained data for volatile components in each plant part gave an insight of the existence of clear and large biodiversity of the chemical profiles of the studied plant parts. The second part of this work was devoted to the phenolic extracts of the flowers part using methanolic solvent. First step, an ultrasound-assisted extraction (UAE) using response surface methodology (RSM) was conducted to optimize the three factors, namely the extraction period, temperature, and sample-to-methanol ratio, on five responses, which were: extraction yield (Y%), Total phenolic content (TPC), Flavonoids content (FC) and two antioxidant activities tests DPPH and FRAP. The optimum conditions were: 50°C for 55 min using sample-to-methanol ratio of 1:70 g/mL (wt/vol). Experimental values were in agreement with the predicted ones, indicating the capability of the model and succeed of RSM in optimizing the extraction conditions. In the second step, the different phenolic classes of the methanolic extracts (TPC, FC and CTC) were quantified at the optimized determined conditions using spectroscopic methods. Subsequently, these extracts were also investigated for their in vitro antioxidant activities using an extended number of assays, namely: DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests. Correlations between these phenolic extracts classes and antioxidant activities were also investigated using Pearson method at level of significance of 0.05.
The quantification of the phenolic extracts results, revealed considerable amounts and variability of TPC (804-544 mg GAE/100 g dm, FC (187-410 mg QE/100 g dm) and CTC (111-394 mg CE/100 g dm). For the antioxidant activities, the most active extracts were corresponding to the following EC50 values of 0.057, 0.005, 0.021, 0.053 and 0.078 (g/L), for the DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests, respectively. Finally, the correlations results showed good correlation between TPC and FC (R2 = 72.49%). Moreover, high correlations were mostly observed between CUPRAC and DPPH, FRAP tests. Finally, this study provided certainly some additional and valuable data about the phytochemical and the chemosystematic characteristics of this plant, which should be useful for the subsequent studies on the pharmacological promising effects of its extracts. | | note de thèses : | Thèse de doctorat en chimie |
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