Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants / Boualem Benamar Aissa

Public ISBD Titre : Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants Type de document : document multimédia Auteurs : Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Ouinten, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2024 Importance : 160 p. Accompagnement : 1 disque optique numérique (CD-ROM) Langues : Anglais Mots-clés : Citrus aurantium  Artemisia herba alba  Artemisia campestris  Essential oils  Phenolic extracts  Synergistic  Antioxidant and biological activities Résumé : The aim of this study was to investigate the combinatory effects of mixtures of extracts from different extracts on antioxidant, antimicrobial, anticholinesterase and anti-α-amylase activities. The extracts involved two different metabolite classes, i.e., essential oils (EOs) and the phenolic extracts (PEs) of plants namely A. campestris (AC), A. herba alba (AHA), and C. aurantium (CA). The EOs obtained from hydrodistilled plant parts were analyzed by GC and GC/MS. Total phenolic content and flavonoid content was evaluated by Folin-Ciocalteu reagent and aluminum chloride colorimetry method, respectively. Antioxidant combination effect was evaluated by DPPH, β-carotene, FRAP and ABTS assays. Antimicrobial combination effect was assessed against five microbial strains using agar diffusion and microbroth dilution techniques. Anticholinesterase and anti-α-amylase combination effect was also tested. The antioxidants activities of EO combinations showed the existence of a very interesting dilution effect of CA for AC and AHA EOs (up to 1/3 vol fraction for DPPH and up to 1/2 for β-carotene). Alternatively, interesting synergistic effects were recorded in the following area of interest for the PEs “0.15 < AC < 0.6 & AHA < 1/3 & 1/3 < CA < 2/3”. The synergistic effect of PEs combinations was observed for the three tested antioxidant activities (DPPH, FRAP and ABTS). The results of anticholinesterase and anti-α-amylase combination showed the existence of synergism and antagonism actions for different binary and tertiary combinations of extracts, especially for the PE. The docking results highlighted several compounds with affinity towards microbial enzymes as well as cholinesterases and α-amylase, suggesting their potential as active compounds. Based on the molecular docking results, a network pharmacological analysis was investigated. The study suggests that the number of compounds from mixtures that interact with target enzymes significantly enhance the antimicrobial, anticholinesterase and anti-α-amylase effects. This research explored the synergistic effects of extracts from different plants and their potential applications in antioxidant, antimicrobial, anticholinesterase and anti-α-amylase. The findings contribute to the understanding of EOs as well as PEs combinations and their beneficial effects in various fields. note de thèses : Thèse de doctorat en sciences biologiques Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants [document multimédia] / Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Ouinten, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 160 p. + 1 disque optique numérique (CD-ROM). Langues : Anglais Mots-clés : Citrus aurantium  Artemisia herba alba  Artemisia campestris  Essential oils  Phenolic extracts  Synergistic  Antioxidant and biological activities Résumé : The aim of this study was to investigate the combinatory effects of mixtures of extracts from different extracts on antioxidant, antimicrobial, anticholinesterase and anti-α-amylase activities. The extracts involved two different metabolite classes, i.e., essential oils (EOs) and the phenolic extracts (PEs) of plants namely A. campestris (AC), A. herba alba (AHA), and C. aurantium (CA). The EOs obtained from hydrodistilled plant parts were analyzed by GC and GC/MS. Total phenolic content and flavonoid content was evaluated by Folin-Ciocalteu reagent and aluminum chloride colorimetry method, respectively. Antioxidant combination effect was evaluated by DPPH, β-carotene, FRAP and ABTS assays. Antimicrobial combination effect was assessed against five microbial strains using agar diffusion and microbroth dilution techniques. Anticholinesterase and anti-α-amylase combination effect was also tested. The antioxidants activities of EO combinations showed the existence of a very interesting dilution effect of CA for AC and AHA EOs (up to 1/3 vol fraction for DPPH and up to 1/2 for β-carotene). Alternatively, interesting synergistic effects were recorded in the following area of interest for the PEs “0.15 < AC < 0.6 & AHA < 1/3 & 1/3 < CA < 2/3”. The synergistic effect of PEs combinations was observed for the three tested antioxidant activities (DPPH, FRAP and ABTS). The results of anticholinesterase and anti-α-amylase combination showed the existence of synergism and antagonism actions for different binary and tertiary combinations of extracts, especially for the PE. The docking results highlighted several compounds with affinity towards microbial enzymes as well as cholinesterases and α-amylase, suggesting their potential as active compounds. Based on the molecular docking results, a network pharmacological analysis was investigated. The study suggests that the number of compounds from mixtures that interact with target enzymes significantly enhance the antimicrobial, anticholinesterase and anti-α-amylase effects. This research explored the synergistic effects of extracts from different plants and their potential applications in antioxidant, antimicrobial, anticholinesterase and anti-α-amylase. The findings contribute to the understanding of EOs as well as PEs combinations and their beneficial effects in various fields. note de thèses : Thèse de doctorat en sciences biologiques

Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants / Boualem Benamar Aissa

Public ISBD Titre : Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants Type de document : texte manuscrit Auteurs : Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse Editeur : University of Amar Telidji - Laghouat Faculty of Science Department of Biology Année de publication : 2024 Importance : 159p Format : 27cm Langues : Anglais Catégories : THESES :14 biologie note de thèses : Memiore doctorat en biologie Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants [texte manuscrit] / Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse . - [S.l.] : University of Amar Telidji - Laghouat Faculty of Science Department of Biology, 2024 . - 159p ; 27cm. Langues : Anglais Catégories : THESES :14 biologie note de thèses : Memiore doctorat en biologie

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Chemical composition of Deverra scoparia Coss. & Durieu essential oils and their phenolic extracts antioxidant activity / Adel Ben Arfa

Public ISBD Titre : Chemical composition of Deverra scoparia Coss. & Durieu essential oils and their phenolic extracts antioxidant activity Type de document : texte manuscrit Auteurs : Adel Ben Arfa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Yousfi, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département des sciences de la matière Année de publication : 2021 Importance : 136 p Format : 27 cm Accompagnement : 1 disque optique numérique (CD-ROM) Note générale : Option : Applied organic chemistry Langues : Anglais Catégories : THESES :06 chimie Mots-clés : Deverra scoparia  Hydro-distillation  Essential oils  Phenolic extracts  Ultrasound-assisted extraction  Response surface methodology  Antioxidant activity Résumé : The widespread use of Deverra scoparia plant (Apiaceous family) in Algerian folk medicine as a remedy, can be relatively attributed to the content of its secondary metabolites. The current study aimed to provide a scientific basis for the potential uses (applications) of this plant extracts in foods (natural antioxidants) and in pharmaceutics industries. This investigation was applied using a large number of randomly collected samples (large scale statistical analysis study) from different regions and locations spreading from semiarid to Saharan territories in Algeria, during two consecutive years (2016 and 2017). Essential oils (EOs) were obtained by hydro-distillation of different parts (flowers and stems) of D. scoparia. EO components were identified using GC and GC/MS. Results showed a considerable variation in the essential oil yields for both studied parts. For the stems part, the EO yield was moderate and varied from 0.48% to 1.68% (vol/wt), while for flowers part it was considerably important and varied in the range of 1.0–4.87% (vol/wt). Seven major compounds identified for the flowers part were varying from very low to very high percentages: α-pinene (2.49–42.32%), sabinene + β-pinene (1.11–30.31%), α-phellandrene (0.99–20.63%), p-cymene (0.0–44.22%), limonene (0.0–42.99%), myristicin (0.03–58.19%) and dill apiole (0.4–25.52%). Similarly, for the stems part, larges variations of both major and minor components were also registered: α-pinene (17.97–53.25%), sabinene + β-pinene (3.44–24.99%), α-phellandrene (0.75–10.32%), limonene (1.35–59.68%), terpinen-4-ol (0.29–15.85%) and spathulenol (0.46–11.29%). Both phenylpropanoids and phthalides classes of bioactive components in leaves part were exhibiting drastic variations: (0.77–59.46%) and (0.04–28.55%), respectively. More specifically, the following bioactive components reached a very high level for this plant species: myristicin “up to 58.19%”, dill apiole “up to 25.52%” and (Z)-ligustilide “up to 27.5%”. Agglomerative Hierarchical Cluster analysis was conducted, showing the existence of two main groups. Obtained data for volatile components in each plant part gave an insight of the existence of clear and large biodiversity of the chemical profiles of the studied plant parts. The second part of this work was devoted to the phenolic extracts of the flowers part using methanolic solvent. First step, an ultrasound-assisted extraction (UAE) using response surface methodology (RSM) was conducted to optimize the three factors, namely the extraction period, temperature, and sample-to-methanol ratio, on five responses, which were: extraction yield (Y%), Total phenolic content (TPC), Flavonoids content (FC) and two antioxidant activities tests DPPH and FRAP. The optimum conditions were: 50°C for 55 min using sample-to-methanol ratio of 1:70 g/mL (wt/vol). Experimental values were in agreement with the predicted ones, indicating the capability of the model and succeed of RSM in optimizing the extraction conditions. In the second step, the different phenolic classes of the methanolic extracts (TPC, FC and CTC) were quantified at the optimized determined conditions using spectroscopic methods. Subsequently, these extracts were also investigated for their in vitro antioxidant activities using an extended number of assays, namely: DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests. Correlations between these phenolic extracts classes and antioxidant activities were also investigated using Pearson method at level of significance of 0.05. The quantification of the phenolic extracts results, revealed considerable amounts and variability of TPC (804-544 mg GAE/100 g dm, FC (187-410 mg QE/100 g dm) and CTC (111-394 mg CE/100 g dm). For the antioxidant activities, the most active extracts were corresponding to the following EC50 values of 0.057, 0.005, 0.021, 0.053 and 0.078 (g/L), for the DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests, respectively. Finally, the correlations results showed good correlation between TPC and FC (R2 = 72.49%). Moreover, high correlations were mostly observed between CUPRAC and DPPH, FRAP tests. Finally, this study provided certainly some additional and valuable data about the phytochemical and the chemosystematic characteristics of this plant, which should be useful for the subsequent studies on the pharmacological promising effects of its extracts. note de thèses : Thèse de doctorat en chimie Chemical composition of Deverra scoparia Coss. & Durieu essential oils and their phenolic extracts antioxidant activity [texte manuscrit] / Adel Ben Arfa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Yousfi, Directeur de thèse . - Laghouat : Université Amar Telidji - Département des sciences de la matière, 2021 . - 136 p ; 27 cm + 1 disque optique numérique (CD-ROM). Option : Applied organic chemistry Langues : Anglais Catégories : THESES :06 chimie Mots-clés : Deverra scoparia  Hydro-distillation  Essential oils  Phenolic extracts  Ultrasound-assisted extraction  Response surface methodology  Antioxidant activity Résumé : The widespread use of Deverra scoparia plant (Apiaceous family) in Algerian folk medicine as a remedy, can be relatively attributed to the content of its secondary metabolites. The current study aimed to provide a scientific basis for the potential uses (applications) of this plant extracts in foods (natural antioxidants) and in pharmaceutics industries. This investigation was applied using a large number of randomly collected samples (large scale statistical analysis study) from different regions and locations spreading from semiarid to Saharan territories in Algeria, during two consecutive years (2016 and 2017). Essential oils (EOs) were obtained by hydro-distillation of different parts (flowers and stems) of D. scoparia. EO components were identified using GC and GC/MS. Results showed a considerable variation in the essential oil yields for both studied parts. For the stems part, the EO yield was moderate and varied from 0.48% to 1.68% (vol/wt), while for flowers part it was considerably important and varied in the range of 1.0–4.87% (vol/wt). Seven major compounds identified for the flowers part were varying from very low to very high percentages: α-pinene (2.49–42.32%), sabinene + β-pinene (1.11–30.31%), α-phellandrene (0.99–20.63%), p-cymene (0.0–44.22%), limonene (0.0–42.99%), myristicin (0.03–58.19%) and dill apiole (0.4–25.52%). Similarly, for the stems part, larges variations of both major and minor components were also registered: α-pinene (17.97–53.25%), sabinene + β-pinene (3.44–24.99%), α-phellandrene (0.75–10.32%), limonene (1.35–59.68%), terpinen-4-ol (0.29–15.85%) and spathulenol (0.46–11.29%). Both phenylpropanoids and phthalides classes of bioactive components in leaves part were exhibiting drastic variations: (0.77–59.46%) and (0.04–28.55%), respectively. More specifically, the following bioactive components reached a very high level for this plant species: myristicin “up to 58.19%”, dill apiole “up to 25.52%” and (Z)-ligustilide “up to 27.5%”. Agglomerative Hierarchical Cluster analysis was conducted, showing the existence of two main groups. Obtained data for volatile components in each plant part gave an insight of the existence of clear and large biodiversity of the chemical profiles of the studied plant parts. The second part of this work was devoted to the phenolic extracts of the flowers part using methanolic solvent. First step, an ultrasound-assisted extraction (UAE) using response surface methodology (RSM) was conducted to optimize the three factors, namely the extraction period, temperature, and sample-to-methanol ratio, on five responses, which were: extraction yield (Y%), Total phenolic content (TPC), Flavonoids content (FC) and two antioxidant activities tests DPPH and FRAP. The optimum conditions were: 50°C for 55 min using sample-to-methanol ratio of 1:70 g/mL (wt/vol). Experimental values were in agreement with the predicted ones, indicating the capability of the model and succeed of RSM in optimizing the extraction conditions. In the second step, the different phenolic classes of the methanolic extracts (TPC, FC and CTC) were quantified at the optimized determined conditions using spectroscopic methods. Subsequently, these extracts were also investigated for their in vitro antioxidant activities using an extended number of assays, namely: DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests. Correlations between these phenolic extracts classes and antioxidant activities were also investigated using Pearson method at level of significance of 0.05. The quantification of the phenolic extracts results, revealed considerable amounts and variability of TPC (804-544 mg GAE/100 g dm, FC (187-410 mg QE/100 g dm) and CTC (111-394 mg CE/100 g dm). For the antioxidant activities, the most active extracts were corresponding to the following EC50 values of 0.057, 0.005, 0.021, 0.053 and 0.078 (g/L), for the DPPH, ABTS, FRAP, CUPRAC and Iron-chelating tests, respectively. Finally, the correlations results showed good correlation between TPC and FC (R2 = 72.49%). Moreover, high correlations were mostly observed between CUPRAC and DPPH, FRAP tests. Finally, this study provided certainly some additional and valuable data about the phytochemical and the chemosystematic characteristics of this plant, which should be useful for the subsequent studies on the pharmacological promising effects of its extracts. note de thèses : Thèse de doctorat en chimie

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Composition chimique, activité antioxydante et antimicrobienne des huiles essentielles de trois espèces végétales du genre Artemisia / Hadjer Aouissi

Public ISBD Titre : Composition chimique, activité antioxydante et antimicrobienne des huiles essentielles de trois espèces végétales du genre Artemisia : Artemisia Absinthium A. Herba Alba et A. Campestris Type de document : texte manuscrit Auteurs : Hadjer Aouissi, Auteur ; Mohamed Ouinten, Directeur de thèse ; Nadhir Gourine, Directeur de thèse Editeur : Laghouat : Université Amar Telidji - Département de biologie Année de publication : 2011 Importance : 90 p. Format : 30 cm. Note générale : Option : Valorisation et connaissance des produits naturels Langues : Français note de thèses : Thèse de magister en sciences biologiques Composition chimique, activité antioxydante et antimicrobienne des huiles essentielles de trois espèces végétales du genre Artemisia : Artemisia Absinthium A. Herba Alba et A. Campestris [texte manuscrit] / Hadjer Aouissi, Auteur ; Mohamed Ouinten, Directeur de thèse ; Nadhir Gourine, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2011 . - 90 p. ; 30 cm. Option : Valorisation et connaissance des produits naturels Langues : Français note de thèses : Thèse de magister en sciences biologiques

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Composition chimique et activité antioxydante de l'huile essentielle du pistachier de l'Atlas (Pistacia atlantica desf) / Nadhir Gourine

Public ISBD Titre : Composition chimique et activité antioxydante de l'huile essentielle du pistachier de l'Atlas (Pistacia atlantica desf) Type de document : texte manuscrit Auteurs : Nadhir Gourine, Auteur ; Mohamed Yousfi, Directeur de thèse ; B. Nadjemi, Directeur de thèse Editeur : Alger : école normal supérieure de Kouba Année de publication : 2010 Importance : 217 p. Format : 30 cm. Note générale : Option : Chimie organique Langues : Français Mots-clés : Pistacia atlantica desf  Huiles essentielles  Composition chimique  Activité antioxydante  pistachier de l'Atlas  Test FRAP  Test DPPH  Chmiotype  ACP  AFD  CAH  Laghhouat  Hassi R'mel  Ain Ouassara note de thèses : Thèse de doctorat en chimie Composition chimique et activité antioxydante de l'huile essentielle du pistachier de l'Atlas (Pistacia atlantica desf) [texte manuscrit] / Nadhir Gourine, Auteur ; Mohamed Yousfi, Directeur de thèse ; B. Nadjemi, Directeur de thèse . - Alger : école normal supérieure de Kouba, 2010 . - 217 p. ; 30 cm. Option : Chimie organique Langues : Français Mots-clés : Pistacia atlantica desf  Huiles essentielles  Composition chimique  Activité antioxydante  pistachier de l'Atlas  Test FRAP  Test DPPH  Chmiotype  ACP  AFD  CAH  Laghhouat  Hassi R'mel  Ain Ouassara note de thèses : Thèse de doctorat en chimie

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Composition chimique,activités antioxydante et antimicrobienne des huiles essentielles de Deverra Scoparia / Malika Maamri

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Composition chimique et activités antioxydante et antimicrobienne des huiles essentielles de quelques plantes de la famille des lamiaceae / Khadidja Houda Benabed

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Dismutation du toluène sur le catalyseur zéolitique FAUJASITE de type Y (FAU-Y) / Hocine Baali

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Effet du climat sur la composition biochimique et analyse de l'action conjuguée de plusieurs mélanges d'extraits naturels de plantes de la région de Laghouat / Imad eddine Kadi

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Étude de l’adsorption du phénol sur la zéolithe de type FAU-X / Khatir Bahaz

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Étude de l’adsorption du phénol sur la zéolithe de type FAU-Y / Soumia Bousmaha

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Étude de l'adsorption de quelques métaux lourds (Nikel et Cobalt) sur la Zéolithe synthétise de type X / Imane Meriem Yacoub

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Étude de l’échange ionique des cations Ca++ sur la zéolithe de type FAU-Y / Bouchra Abdi

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Étude de l’échange ionique des ions K+ et NH4+ en utilisant la zéolithe de type Na X / Latifa Boukanoun

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Étude de l'échange ionique des ions K+ sur la zéolithe de type Na-Y / Malika Taifouri

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