| Titre : | Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants | | Type de document : | document multimédia | | Auteurs : | Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Ouinten, Directeur de thèse | | Editeur : | Laghouat : Université Amar Telidji - Département de biologie | | Année de publication : | 2024 | | Importance : | 160 p. | | Accompagnement : | 1 disque optique numérique (CD-ROM) | | Langues : | Anglais | | Mots-clés : | Citrus aurantium Artemisia herba alba Artemisia campestris Essential oils Phenolic
extracts Synergistic Antioxidant and biological activities | | Résumé : | The aim of this study was to investigate the combinatory effects of mixtures of extracts from different extracts on antioxidant, antimicrobial, anticholinesterase and anti-α-amylase activities.
The extracts involved two different metabolite classes, i.e., essential oils (EOs) and the phenolic extracts (PEs) of plants namely A. campestris (AC), A. herba alba (AHA), and C. aurantium (CA). The EOs obtained from hydrodistilled plant parts were analyzed by GC and GC/MS. Total phenolic content and flavonoid content was evaluated by Folin-Ciocalteu reagent and aluminum chloride colorimetry method, respectively. Antioxidant combination effect was evaluated by DPPH, β-carotene, FRAP and ABTS assays. Antimicrobial combination effect was assessed against five microbial strains using agar diffusion and microbroth dilution techniques. Anticholinesterase and anti-α-amylase combination effect was also tested. The antioxidants activities of EO combinations showed the existence of a very interesting dilution effect of CA for AC and AHA EOs (up to 1/3 vol fraction for DPPH and up to 1/2 for β-carotene). Alternatively, interesting synergistic effects were recorded in the following area of interest for the PEs “0.15 < AC < 0.6 & AHA < 1/3 & 1/3 < CA < 2/3”. The synergistic effect of PEs combinations was observed for the three tested antioxidant activities (DPPH, FRAP and ABTS). The results of anticholinesterase and anti-α-amylase combination showed the existence of synergism and antagonism actions for different binary and tertiary combinations of extracts, especially for the PE.
The docking results highlighted several compounds with affinity towards microbial enzymes as well as cholinesterases and α-amylase, suggesting their potential as active compounds. Based on the molecular docking results, a network pharmacological analysis was investigated. The study suggests that the number of compounds from mixtures that interact with target enzymes significantly enhance the antimicrobial, anticholinesterase and anti-α-amylase effects. This research explored the synergistic effects of extracts from different plants and their potential applications in antioxidant, antimicrobial, anticholinesterase and anti-α-amylase. The findings contribute to the understanding of EOs as well as PEs combinations and their beneficial effects in various fields. | | note de thèses : | Thèse de doctorat en sciences biologiques |
Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants [document multimédia] / Boualem Benamar Aissa, Auteur ; Nadhir Gourine, Directeur de thèse ; Mohamed Ouinten, Directeur de thèse . - Laghouat : Université Amar Telidji - Département de biologie, 2024 . - 160 p. + 1 disque optique numérique (CD-ROM). Langues : Anglais | Mots-clés : | Citrus aurantium Artemisia herba alba Artemisia campestris Essential oils Phenolic
extracts Synergistic Antioxidant and biological activities | | Résumé : | The aim of this study was to investigate the combinatory effects of mixtures of extracts from different extracts on antioxidant, antimicrobial, anticholinesterase and anti-α-amylase activities.
The extracts involved two different metabolite classes, i.e., essential oils (EOs) and the phenolic extracts (PEs) of plants namely A. campestris (AC), A. herba alba (AHA), and C. aurantium (CA). The EOs obtained from hydrodistilled plant parts were analyzed by GC and GC/MS. Total phenolic content and flavonoid content was evaluated by Folin-Ciocalteu reagent and aluminum chloride colorimetry method, respectively. Antioxidant combination effect was evaluated by DPPH, β-carotene, FRAP and ABTS assays. Antimicrobial combination effect was assessed against five microbial strains using agar diffusion and microbroth dilution techniques. Anticholinesterase and anti-α-amylase combination effect was also tested. The antioxidants activities of EO combinations showed the existence of a very interesting dilution effect of CA for AC and AHA EOs (up to 1/3 vol fraction for DPPH and up to 1/2 for β-carotene). Alternatively, interesting synergistic effects were recorded in the following area of interest for the PEs “0.15 < AC < 0.6 & AHA < 1/3 & 1/3 < CA < 2/3”. The synergistic effect of PEs combinations was observed for the three tested antioxidant activities (DPPH, FRAP and ABTS). The results of anticholinesterase and anti-α-amylase combination showed the existence of synergism and antagonism actions for different binary and tertiary combinations of extracts, especially for the PE.
The docking results highlighted several compounds with affinity towards microbial enzymes as well as cholinesterases and α-amylase, suggesting their potential as active compounds. Based on the molecular docking results, a network pharmacological analysis was investigated. The study suggests that the number of compounds from mixtures that interact with target enzymes significantly enhance the antimicrobial, anticholinesterase and anti-α-amylase effects. This research explored the synergistic effects of extracts from different plants and their potential applications in antioxidant, antimicrobial, anticholinesterase and anti-α-amylase. The findings contribute to the understanding of EOs as well as PEs combinations and their beneficial effects in various fields. | | note de thèses : | Thèse de doctorat en sciences biologiques |
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Analysis of the combined antioxidant and biological activities of mixtures of natural extracts from some local wild plants / Boualem Benamar Aissa
